HNBGU BSc CBZ Chemistry 5th Sem Syllabus 2022-23

Theory: 60 Lectures
1. Qualitative and quantitative aspects of analysis: Sampling,
evaluation of analytical data, errors, accuracy, and precision, methods
of their expression, normal law of distribution if indeterminate errors,
statistical test of data; F, Q and test, rejection of data, and confidence intervals.
(5 Lectures)

2. Optical methods. of analysis: Origin of spectra, the interaction of
radiation with matter, fundamental laws of spectroscopy and selection
rules, the validity of Beer-Lamberts law.
* UV-Visible Spectrometry: Basic principles of instrumentation (choice
of source monochromator and detector) for single and double beam
instrument
* Basic principles of quantitative analyst estimation of metal ions from
the aqueous solution, geometrical isomers, keto-enol tautomers.
* Determination of the composition of metal complexes using Job’s method
continuous variation and mole ratio method.
* Infrared Spectrometry: Basic principles of instrumentation (choice of
source, monochromator & detector) for single and double beam
instrument; sampling techniques.
* Structural illustration through interpretation of data, Effect, and
importance of isotope substitution.
* Flame Atomic Absorption and Emission Spectrometry: Basic
principles’ of instrumentation (choice of source, monochromator,
detector, choice of flame, and Burner designs. Techniques of
atomization and sample introduction; Method of background
correction, sources of chemical interferences, and their method of
removal. Techniques for the quantitative estimation of trace levels of
metal ions from water samples.
(25 Lectures)

3. Thermal methods of analysis: Theory of thermogravimetry (TG),
the basic principle of instrumentation. Techniques for quantitative
estimation of Ca and Mg from their mixture. (5 Lectures)

4. Electroanalytical methods: Classification of electroanalytical methods,
the basic principle of pH metric, potentiometric and conductometric
titrations. Techniques used for the determination of equivalence points.
Techniques used for the determination of pKa values. (10 Lectures)

5. Separation techniques: Solvent extraction: Classification, principles,
and efficiency of the technique.
* Mechanism of extraction: Extraction by solvation and chelation.
* The technique of extraction: Batch, continuous, and counter-current
extractions. Qualitative and quantitative aspects of solvent extraction:
extraction of metal ions from aqueous solution, extraction of organic
species from the aqueous and nonaqueous media.

-Chromatography: Classification, principles, and efficiency of the technique.
Mechanism of Separation: adsorption, partition, and ion exchange.
* Development of Chromatograms: frontal, elution, and displacement methods.
* Qualitative and quantitative aspects of chromatographic methods
of analysis: IC, GLC, GPC, TLC, and HPLC. Stereoisomeric separation
and analysis: reagents. Chiral Measurement of optical rotation, calculation
of Enantiomeric excess (cc)/ diastereomeric excess (de) ratios, and
determination of enantiomeric composing using NMR, Chiral solvents,
and chiral shift chromatographic techniques using chiral columns (GC and HPLC).
* Role of computers in instrumental methods of analysis. (15 Lectures)